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>We are struggeling with intracellular staining of INFg/IL4 using BD
>FASTimmune cytokine system.
>If anyone has succeded -what is the trick?
One trick I recall hearing is that for the whole blood assay the peripheral
blood must be collected in heparin tubes not EDTA as the cytokine production
is a Ca+ dependent response. Good luck.
-------------------------------------
Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Cornell University Medical College
Manhasset, Long Island, New York
ph: 516-562-4641 fax: 516-562-2866
e-mail: thomasm@nshs.edu
Date: 12/10/96 Time: 15:40:21
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web