Ethanol fixation
Bruce S. Hass (BHASS@NCTR.FDA.GOV)
Tue, 10 Sep 1996 11:54:29 CST
Hi,
I am interested in staining cells with Bodipy by the method of Gocze
and Freeman (Cytometry 17:151-158 1994), which calls for ethanol
fixed cells. Unfortunately the paper does not give details about the
fixation procedure. Is ethanol fixation so simple, obvious, or common?
Any suggestions on how to do it would be appreciated. Thanks.
Bruce Hass
National Center for Toxicological Research
Jefferson AR 72079
(501) 543 7365
(501) 543 7745 (fax)
bhass@nctr.fda.gov
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu