CY5

Deborah Berglund (umbbd@gemini.oscs.montana.edu)
Fri, 11 Oct 1996 12:50:33 -0600 (MDT)

Hi everyone,

We are observing something very wierd about CY5 fluorescence. It seems
that the surface labelling of an receptor with a biotinylated peptide with
secondary avidin CY5 is the right fluorescence wavelengths, but when the
whole receptor (we think) is internalized, the fluorescence
characteristics of CY5 change, the fluorescence appears in the same
excitation and emission filters as CY3. Anyone else ever observe this?
We are not doing flow, but using confocal microscopy.

Thanks

Deb Berglund
Montana State University

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu