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I am now in the process of writing up a chapter for the Current Protcols in
Cytometry. As it happens, I am just done with "the mitochondria".
If you want to quantify mitochondria per se you best use MitoTracker Green
(Molecular Probes, Inc. # 7514). This dye is 488 nm excitable and gives
bright (and narrow) green fluorescence. Another dye from Molecular Probes,
CMXRos (# 7512) gives red fluorescence and responds to changes in
mitochondrial membrane potential. Both dyes are retained after fixation
with aldehydes.
I do not recommend rhodamine 123, because its cell staining is so
photo-unstable; JC-1 does funny things in my hands and appears to give a lot
of red background staining outside of the mitochondria. Molecular Probes,
Inc. may be able to give you more information (Tel: 541-465-8300; FAX:
541-344-6504).
I have no idea as to how Red 613 relates to mitochondria.
Hope this helps,
Martin Poot
martin@probes.mhs.compuserve.com
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