This material was originally published in the Purdue Cytometry CD-ROM Series
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Flow Cytometry and Microbiology



Combination of flow cytometry and fluorescent probes to determine bacterial viability.


Roger W. Pickup
Institute of Freshwater Ecology
The Ferry House
Far Sawry
Ambleside, UK
email: ROGER@wpo.nerc.ac.uk

Jonathan P. Porter
Department of Genetics and Microbiology
Life Sciences Department
University of Liverpool
Liverpool, L69 3BX, UK
email: JOPO@wpo.nerc.ac.uk

Data Examples

Fluorescent Probes

Porter, J.P., C. Edwards, and R.W. Pickup. 1995. Rapid assessment of physiological status of Escherichia coli using fluorescent probes. Journal of Applied Bacteriology 79:399-408. [reproduced with permission]


Membrane Potential Dyes

Deere, D., J.P. Porter, R.W. Pickup, and C. Edwards. 1995. Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid)trimethine oxonol, (diBA-C4(3)-), for the flow cytometric assessment of bacterial viability. FEMS Microbiology Letters 130:165-170. [reproduced with permission]


Enzymatic Activity

Porter, J., J. Diaper, C. Edwards, and R.W. Pickup. 1995. Direct viability measurements of natural planktonic bacterial communities. Applied and Environmental Microbiology 61:2783-2786. [reproduced with permission]


Acridine Orange
Morgan, J.A.W., K.J. Clark, G. Rhodes, and R.W. Pickup. 1992. Non-culturable Aeromonas salmonicida in lakewater. Microbial Releases 1: 71-78. [reproduced with permission]


Survival Studies
Pickup, R.W., G.Rhodes, R.J. Cobban, and K.J. Clarke. 1996. Postponement of non-culturability in Aeromonas salmonicida. Journal of Fish Diseases 19:65-74. [reproduced with permission]


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