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CONTENTS |
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Contents of Volume 42 |
xvii |
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Contributors |
xxi |
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Preface to the Second Edition |
xxvii |
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Preface to the First Edition |
xxxi |
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1. A Flow Cytometric Technique for Detection of DNA Repair in Mammalian Cells |
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Jules R. Selden and Frank Dolbeare |
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I. Introduction |
1 |
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II. Application |
3 |
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III. Materials |
4 |
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IV. Sample Preparation and Staining |
5 |
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V. Critical Aspects ofthe Procedure |
9 |
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VI. Controls and Standards |
11 |
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VII. Instruments and Setup for Data Collection |
12 |
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VIII. Results and Discussion |
13 |
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References |
18 |
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2. Rapid Determination of Cellular Resistance-Related Drug Efflux in Tumor Cells |
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Awtar Krishan |
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I. Introduction |
21 |
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II. Application |
22 |
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III. Materials |
23 |
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IV. Staining Procedure |
24 |
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V. Critical Aspects |
24 |
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VI. Controls and Standards |
25 |
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VII. Instruments |
25 |
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VIII. Results |
26 |
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References |
30 |
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3. Glutathione and Cellular Resistance to Anti-Cancer Drugs |
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David Hedley and Sue Chow |
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I. Introduction |
31 |
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II. Flow Cytometric Glutathione Probes |
33 |
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III. Materials |
36 |
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IV. Methods |
37 |
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References |
44 |
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4. Assay of Cell Resistance to Ara-C |
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Francis Lacombe |
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I. Introduction |
45 |
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II. Application |
47 |
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III. Materials |
48 |
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IV. Cell Preparation and Staining |
48 |
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V. Critical Aspects of the Procedure |
49 |
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VI. Instruments |
49 |
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VII. Results and Discussion |
52 |
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VIII. Comparison of the Methods |
56 |
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References |
57 |
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5. Detection of mRNA Species by Flow Cytometry |
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Francis Belloc and Francoise Durrieu |
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I. Introduction |
59 |
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II. Applications |
61 |
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III. Materials |
62 |
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IV. Staining Procedures |
63 |
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V. Critical Aspects of the Procedure and Controls |
64 |
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VI. Instruments and FCM Analysis |
66 |
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VII. Results and Discussion |
67 |
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References |
68 |
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6. Primed in Situ Labeling (PRINS) and Fluorescence in Situ Hybridization (FISH) |
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Hanne Fischer, Johnny Hindkjoer, Soren Pedersen, Jorn Koch, Carsten Brandt, and Steen K0luroa |
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I. Introduction |
72 |
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II. Applications |
72 |
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III. Materials |
73 |
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IV. Protocols |
76 |
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V. Critical Aspects of the Procedures |
87 |
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VI. Instruments |
89 |
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VII. Results and Discussion |
90 |
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VIII. Comparison of Methods |
92 |
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References |
93 |
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7. Molecular Phenotyping by Flow Cytometry |
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Benjamin D. Li, Earl A. Timm, Jr., Mary C. Riedy, Seth P. Harlow, and Carleton C. Stewart |
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I. Introduction |
96 |
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II. General Materials and Methods |
97 |
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III. Measurement of Gene Amplification Using Competitive PCR |
101 |
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IV. Measurement of Gene Expression Using Competitive RT-PCR |
115 |
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V. Fluorescence in Situ Hybridization En Suspension (FISHES) |
121 |
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VI. Fluorescence in Situ PCR (FLIP) |
126 |
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References |
129 |
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8. Isolation and Analysis of Somatic Cell Mutants with Defects in Endocytic Traffic |
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Sandra A. Brockman and Robert F. Murphy |
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I. Introduction: Why Isolate Somatic Cell Mutants in Membrane Traffic? |
131 |
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II. General Considerations |
133 |
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III. Isolation of CHO Cells Defective in Receptor Recycling |
139 |
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IV. Isolation of B Cells with Reduced Cysteine Protease Activity 142 |
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V. Conclusions |
146 |
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References |
147 |
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9. Measurement of Micronuclei by Flow Cytometry |
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Michael Nusse, Wolfgang Beisker, Johannes Kramer, Beate M. Miller, Georg A. Schreiber, Silvia Viaggi, Eva Maria Weller, and Jurina M. Wessels |
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I. Introduction |
149 |
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II. Application |
150 |
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III. Materials |
151 |
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IV. Cell Preparation and Staining |
152 |
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V. Critical Aspects ofthe Procedure |
152 |
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VI. Standards |
153 |
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VII. Instruments |
154 |
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VIII. Results and Discussion |
155 |
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References |
158 |
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10. Sperm Chromatin Structure Assay: DNA Denaturability |
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Donald Evenson and Lorna Jost |
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I. Introduction |
160 |
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II. Applications ofthe SCSA |
161 |
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III. Materials |
163 |
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IV. Cell Preparation |
164 |
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V. Cell Staining and Measurement |
166 |
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VI. Instruments |
168 |
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VII. Results and Discussion |
169 |
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VIII. Critical Points |
173 |
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References |
1 75 |
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11. Fine-Needle Cytopuncture and Flow Cytometry in the Characterization of Human Tumors |
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Frederique Spyratos and Marianne Brifford |
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I. Introduction |
178 |
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II. Materials and Methods |
178 |
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III. Critical Aspects |
181 |
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IV. Alternative Methods Applied to Cytopunctures |
182 |
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V. Clinical Applications |
187 |
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VI. Conclusion |
191 |
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References |
192 |
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Note Added in Proof |
192 |
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12. Functional NK Assays Using Flow Cytometry |
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Stefano Papa and Massimo Valentini |
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I. Introduction |
194 |
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II. Application |
196 |
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III. Materials |
196 |
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IV. Cell Preparation and Staining |
197 |
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V. Critical Aspects ofthe Procedures |
198 |
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VI. Standardization |
200 |
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VII. Instruments |
200 |
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VIII. Results and Discussion |
201 |
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References |
206 |
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13. Antibodies to Intermediate Filament Proteins as Probes for Multiparameter Flow Cytometry of Human Cancers |
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T. Vincent Shankey |
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I. Introduction |
209 |
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II. Intermediate Filament Proteins |
210 |
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III. Cell Isolation and Fixation |
214 |
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IV. Multiparameter Flow Cytometric Analysis Using Antibodies |
216 |
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to Cytokeratins |
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V. Clinical Application Using Multiparameter DNA Content |
222 |
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Flow Cytometry |
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VI. Conclusions |
227 |
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References |
228 |
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14. Interactive Data Analysis for Evaluation of B-Cell Neoplasia by Flow Cytometry |
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Ricardo E. Duque |
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I. Background |
231 |
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II. Correlated Multiparametric Data Analysis of B-Cell Neoplasia |
235 |
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References |
241 |
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15. Analysis and Sorting of Hematopoietic Stem Cells from Mouse Bone Marrow |
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Jan W. M. Visser and Peter de Vries |
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I. Introduction |
244 |
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II. Application |
247 |
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III. Materials |
248 |
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IV. Instruments |
250 |
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V. Staining and Sorting Procedure |
250 |
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VI. Critical Aspects of the Procedure |
255 |
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VII. Results and Discussion |
257 |
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References |
260 |
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16. Reticulocyte Analysis and Reticulocyte Maturity Index |
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Bruce H. Davis and Nancy C. Bigelow |
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I. Introduction |
263 |
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II. Reticulocyte Maturity Index |
266 |
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III. Data Analysis |
267 |
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IV. Quality Control |
270 |
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V. Method for Thiazole Orange Reticulocyte Analysis |
271 |
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References |
273 |
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17. Analysis of Platelets by Flow Cytometry |
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Kenneth A. Ault and fane Mitchell |
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I. Introduction |
275 |
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II. Applications |
277 |
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III. Materials |
279 |
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IV. Cell Preparation |
280 |
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V. Staining |
283 |
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VI. Critical Aspects |
285 |
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VII. Standards |
286 |
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VIII. Instrument |
287 |
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IX. Results and Discussion |
287 |
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References |
293 |
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18. Flow Cytometry in Malaria Detection |
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Chris J. Fanse and Philip H. Van Vianen |
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I. Introduction |
296 |
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II. Applications |
297 |
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III. Materials |
300 |
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IV. Cell Preparation and Staining |
300 |
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V. Critical Aspects of the Preparation and Staining Procedures |
304 |
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VI. Standards |
305 |
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VII. Instruments |
305 |
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VIII. Results and Discussion |
307 |
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IX. Comparison of Methods |
316 |
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References |
318 |
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19. Large-Scale Chromosome Sorting |
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John J. Famcett, Jonathan L. Longmire, John C. Martin, Larry L. Deaven, and L. Scott Cram |
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I. Introduction |
319 |
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II. Sample Preparation |
320 |
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III. Preparation Evaluation |
324 |
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IV. Production Chromosome Sorting |
325 |
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Appendix: Polyamine Sheath Buffer |
328 |
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References 329 |
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20. Strategies for Rare Cell Detection and Isolation |
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James F. Leary |
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I. Introduction |
332 |
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II. Methods |
341 |
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III. Critical Aspects of the Procedure |
348 |
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IV. Instrumentation |
354 |
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V. Results: Some Rare Cell Applications and Strategies |
356 |
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VI. Discussion |
357 |
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References |
358 |
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21. Cell Sorting of Biohazardous Specimens for Assay of Immune Function |
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Janis V. Giorgi |
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I. Introduction |
359 |
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II. Applications |
360 |
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III. Critical Aspects of the Procedure |
360 |
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IV. Instruments |
362 |
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V. Verification of Aerosol Containment |
363 |
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VI. Staining and Cell Separation of Potentially Biohazardous Specimens |
367 |
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VII. Results and Discussion |
368 |
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VIII. Alternate Technologies for Cell Separation |
368 |
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References |
369 |
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22. High-Speed Photodamage Cell Sorting: An Evaluation of the |
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ZAPPER Prototype |
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Jan F. Keij, Ad C. Groenewegen, andJan W. M. Visser |
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I. Introduction |
372 |
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II. Application |
373 |
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III. Materials |
373 |
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IV. Critical Aspects of the Procedure |
377 |
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V. Instrumentation |
381 |
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VI. Results and Discussion |
382 |
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References |
385 |
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23. High-Gradient Magnetic Cell Sorting |
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Andreas Radbruch, Birgit Mechtold, Andreas Thiel, Stefan Miltenyi, and Eckhard Pfluger |
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I. Introduction |
388 |
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II. Application |
388 |
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III. Materials |
390 |
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IV. MACS: Staining and Sorting |
391 |
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V. Critical Aspects of the Procedure |
395 |
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VI. Controls |
397 |
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VII Instruments |
397 |
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VIII. Results |
399 |
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References |
402 |
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24. Contributions of Flow Cytometry to Studies with Multicell Spheroids |
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Ralph E. Durand |
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I. Introduction |
406 |
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II. Spheroid Growth and Analysis |
406 |
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III. Flow Cytometry Techniques |
407 |
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IV. Studies of Spheroid Oxygenation |
408 |
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V. Evaluation of Antineoplastic Drugs |
410 |
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VI. Drug Interactions: Synergy in Cell Subpopulations |
414 |
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VII. Multifraction Treatments |
415 |
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VIII. Cell Kinetics: Role of Pretreatment Kinetics |
417 |
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IX. Alternatives for Kinetic Measurements: Overcoming Heterogeneity |
419 |
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X. General Conclusions |
420 |
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References |
421 |
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25. Functional Measurements Using HL-60 Cells |
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J. Paul Robinson, Padma Kumar Narayanan, and Wayne 0. Carter |
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I. Introduction |
423 |
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II. Application |
424 |
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III. Materials and Methods |
425 |
|
IV. Results and Discussion |
431 |
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References |
436 |
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26. HIV Infection: Diagnosis and Disease Progression Evaluation |
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Janis V. Giorgi and Alan Landay |
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I. Introduction |
438 |
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II. Application |
439 |
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III. Materials |
439 |
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IV. Cell Preparation and Staining |
442 |
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V. Critical Aspects of the Procedure |
445 |
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VI. Controls and Standards |
450 |
|
VII. Instruments |
452 |
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VIII. Results and Discussion |
452 |
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IX. Alternative Technologies |
453 |
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References |
454 |
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27. Cell-Cycle Analysis of Saccharomyces cerevisiae |
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Bruce S. Dien, Marvin S. Peterson, and Friedrich Srienc |
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I. Introduction |
458 |
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II. Materials |
460 |
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III. Methods |
461 |
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IV. Critical Aspects |
463 |
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V. Flow Cytometry |
465 |
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VI. Analysis of DNA Histograms |
466 |
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VII. Results |
466 |
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References |
474 |
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28. Staining and Measurement of DNA in Bacteria |
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Harald B. Steen, Mette W. Jernaes, Kirsten Skarstad, and Erik Boye |
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I. Introduction |
477 |
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II. Materials |
480 |
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III. Fixation and Staining |
481 |
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IV. Vital Staining |
481 |
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V. Flow Cytometric Measurement |
|
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VI. Standards and Controls |
484 |
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References |
487 |
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29. Detection of Specific Microorganisms in Environmental Samples Using Flow Cytometry |
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Graham Vesey, foe Narai, Nicholas Ashbolt, Keith Williams, and Duncan Veal |
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I. Introduction |
490 |
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II. Preparation of Water Samples for Flow Cytometric Analysis |
499 |
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III. Staining of Organisms from Water Samples for Flow |
501 |
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Cytometric Analysis |
|
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IV. Flow Cytometric Analysis of Water Samples |
509 |
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V. Instrumentation Developments for Environmental Applications |
516 |
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VI. The Future of Flow Cytometry within Environmental Microbiology |
518 |
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References |
519 |
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30. Strategies for Flow Cytometric Analysis of Marine Microalgae and Sponge Cells |
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Clarice M. Yentsch and Shirley A. Pomponi |
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I. Introduction |
524 |
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II. Application |
524 |
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III. Materials |
526 |
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IV. Cell Preparation and Staining |
526 |
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V. Instrumentation |
532 |
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VI. Results |
536 |
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VII. Discussion |
537 |
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References |
538 |
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31. Flow Cytometry and Sorting of Plant Protoplasts and Cells |
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David W. Galbraith |
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I. Introduction |
540 |
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II. Application |
542 |
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III. Materials |
542 |
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IV. Procedures |
543 |
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V. Critical Aspects of the Procedures |
550 |
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VI. Controls and Standards |
555 |
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VII. Instruments |
555 |
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VIII. Results |
556 |
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References |
560 |
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32. Use of Fluorescence-Activated Cell Sorting for Rapid Isolation of Insect Cells Harboring Recombinant Baculovirus |
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Maja A. Sommerfelt and Eric J. Sorscher |
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I. Introduction |
564 |
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II. Application |
565 |
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III. Materials |
565 |
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IV. Methods |
566 |
|
V. Critical Aspects of the Procedure |
570 |
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VI. Instruments |
570 |
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VII. Results and Discussion |
570 |
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VIII. Comparison of Methods |
572 |
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References |
573 |
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33. Flow Microsphere Immunoassay for the Quantitative and Simultaneous Detection of Multiple Soluble Analytes |
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Thomas M. McHugh |
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I. Introduction |
575 |
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II. Instrumentation and Materials |
578 |
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III. Procedures |
580 |
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IV. Review of Published Applications |
590 |
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V. Summary |
594 |
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VI. Suppliers |
594 |
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References |
595 |
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34. Calibration of Flow Cytometer Detector Systems |
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Ralph E. Durand |
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| |
|
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I. Introduction |
597 |
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II. Materials |
598 |
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III. Procedures |
599 |
|
IV. Concluding Comments |
603 |
|
References |
604 |
| |
|
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35. Standardization for Flow Cytometry |
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A. Schwartz and Emma Fernandez-Repollet |
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|
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I. Introduction |
605 |
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II. Considerations for Qualitative Standardization |
606 |
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III. Considerations for Quantitative Standardization |
619 |
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IV. Conclusions |
624 |
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References |
625 |
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36. Phase-Sensitive Detection Methods for Resolving Fluorescence Emission Signals and Directly Quantifying Lifetime |
|
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John A. Steinkamp |
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| |
|
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I. Introduction |
627 |
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II. Application |
632 |
|
III. Cell Preparation and Staining |
633 |
|
IV. Critical Aspects ofthe Procedure |
633 |
|
V. Standards |
635 |
|
VI. Instrumentation |
635 |
|
VII. Results and Discussion |
636 |
|
References |
640 |
| |
|
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37. Spectra of Fluorescent Dyes Used in Flow Cytometry |
|
|
Richard P. Haugland |
|
| |
|
|
I. Introduction |
641 |
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II. Spectral Properties of Fluorescent Dyes |
642 |
|
III. Immunofluorescence and Related Applications |
645 |
|
IV. Nucleic Acid Stains |
652 |
|
V. Probes for Live Cell Physiology |
655 |
|
VI. Summary |
661 |
|
References |
662 |
| |
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Index |
665 |
|
Volumes in Series |
693 |
